Tumor is palped and its margins are observed with the tangential light, which show its edges. A line is drawn around its perimeter using a topical steryl colorant pencil.
We eliminate the affected area macroscopically (debulking) with a superficial horizontal cut of the affected area. The three-dimensional image of the basal cell carcinoma helps determine the nature of the invasion of this tumor over healthy tissue.
We initiate the technique two millimeters away from the excised area using a scalpel with an inclination of 45 degrees including epidermis and the rear face of the tumor on a single level/plane to avoid an uneven surface.
The immediate histological examination of the tumor and all the area where the tumor was resting, as if being an open book, allows us to examine all of the histological area without leaving any room to leave behind any malignant cells.
In order to achieve this there has to be a photographic match from the extracted tissue with the perform map where the tumor tissue is deposited, respecting the same orientation. The perimeter of the “piece” is marked with red and black dyes. These dyes, along with the epidermal curb, allow us to locate on a microscopic level any possible malignant cells.
A cut is performed, with a thickness of 5 microns beginning from the back of the piece. Whether being a basal cell or a scaly cell carcinoma, it is introduced into the cryostat, under 20 ° C and stained with a special blue tincture (toluidine), whether it is a basal cell or a scally cell carcinoma.